Figure 6: Npn1 and Npn2 invalidation alters the orientation of neural progenitor divisions at E10.5.

(a) Confocal images showing immunolabelling of Npn1 and Npn2 on spinal cord sections at E10.5. Npn1 and Npn2 proteins are distributed in complementary domains of the VZ. Ctrl-immunostaining without primary antibody. (b) Cumulative fraction curves showing the distribution of neural progenitor division orientation in E10.5 Npn1sema/+;Npn2+/− and Npn1sema/sema;Npn2−/− spinal cords (n, number of cells from a minimum of three embryos per conditions from the same litters, P value is from the KS test). The cumulative curve of the E10.5 Sema3B+/+condition has been added for comparison. Previous data sets are also displayed in pie charts (P value is from Fisher’s exact test).