Figure 2: Fluorescein-conjugated macrocycles localized on the cellular membranes of live cells. | Nature Communications

Figure 2: Fluorescein-conjugated macrocycles localized on the cellular membranes of live cells.

From: Artificial human Met agonists based on macrocycle scaffolds

Figure 2

EHMES-1 cells transiently overexpressing Met were treated with (a–d) Hoechst33342 and macrocycle-Fluo as follows: (e) aML5-Fluo, (f) aMD4-Fluo, (g) aMD5-Fluo, or (h) aMsD4-Fluo. (i–l) Cell surface Met stained with an anti-Met rat mAb and an anti-rat mAb-Alexa-594 conjugate. (m–p) Superimposed images of macrocycle-Fluo (green) and anti-Met mAb (red) fluorescent signals. Scale bars, 20 μm.

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