Figure 4: Defective autophagosome maturation in Irf8−/− MФs. | Nature Communications

Figure 4: Defective autophagosome maturation in Irf8−/− MФs.

From: IRF8 directs stress-induced autophagy in macrophages and promotes clearance of Listeria monocytogenes

Figure 4

(a) Immunostaining of LAMP2 in WT and Irf8−/− MФs stimulated with IFNγ overnight and TLR ligands for 8 h. Cells were counterstained for DNA (blue). Scale bar, 20 μm. (b) Immunoblot detection of LAMP2 in WT and Irf8−/− MФs. Ten micrograms of extracts were tested with antibody against LAMP2 or β-Tubulin. Below: the amounts of LAMP2 in three independent experiments were quantified using the ImageJ software. **P≤0.01. (c) WT and Irf8−/− MФs were immunostained for LC3 antibody and LysoTracker-red to detect LC3-positive and lysosome-positive structures. Right: cells with double-positive vesicles were quantified as in Fig. 3a. Data represent the average of three independent experiments. P≤0.01 (**). Scale bar, 20 μm.

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