Figure 2: BN excludes all substrates of ThrRS for protein translation.
From: Structural basis for full-spectrum inhibition of translational functions on a tRNA synthetase
(a) Section view of BN (orange) occupying the catalytic site cavity of ThrRS. (b) Close-up view of L-Thr pocket of superimposed E. coli ThrRS–L-Thr structure (blue, PDB: 1EVK) with ThrRS–BN structure (cyan). Numbering of residues is according to human ThrRS and the corresponding E. coli ThrRS residues are shown in parenthesis. (c) Close-up view of ATP pocket of superimposed Staphylococcus aureus ThrRS–ATP structure (magenta, PDB: 1NYR) with ThrRS–BN structure (cyan). (d) Close-up view of tRNA pocket of superimposed E. coli ThrRS–tRNAThr structure (yellow, PDB: 1QF6) with ThrRS–BN structure (cyan). (e) Close-up view of extra hydrophobic pocket occupied by BN (orange), which is beyond the substrate-binding pockets.
