Figure 5: YAP overexpression or Nf2/WWC1 depletion restores osteopheres in Sox2-depleted cells. | Nature Communications

Figure 5: YAP overexpression or Nf2/WWC1 depletion restores osteopheres in Sox2-depleted cells.

From: Sox2 antagonizes the Hippo pathway to maintain stemness in cancer cells

Figure 5

(a) Western blot showing levels of Sox2 expression in mOS-482–shSox2 cells. (b) Western analysis of YAP expression in shSox2 cells transduced with a control or YAP lentivirus (left panel). Relative sphere-forming efficiency of YAP-transduced shSox2 cells compared with control (parental) mOS-482 cells (right panel). (c) Western analysis of YAP and Nf2 expression in shSox2 cells transfected with scrambled (scr) or two independent Nf2 siRNAs (left panel). Relative sphere-forming efficiency of Nf2-depleted shSox2 cells compared with control (parental) mOS-482 cells (right panel). (d) Western analysis of YAP and WWC1 expression in shSox2 cells transfected with scrambled (scr) or two independent WWc1 siRNAs (left panel). Relative sphere-forming efficiency of WWC1-depleted shSox2 cells compared with control (parental) mOS-482 cells (right panel). *P<0.05 by ANOVA. Error bars represent mean ± s.d.

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