Figure 8: The Sox2-Hippo axis is conserved in multiple cell types.
From: Sox2 antagonizes the Hippo pathway to maintain stemness in cancer cells
(a) Upper panel: western analysis of YAP, WWC1, Nf2 and Sox2 in NIH-3T3 cells (control, or Nf2 shRNA-A lentivirus or Sox2 lentivirus). Lower panel: colony assay of NIH-3T3 cells expressing the indicated constructs. Colonies were stained with Crystal violet and counted after 10 days. (b) Primary glioblastoma multiforme (GBM) cells were transduced with a control or Sox2 shRNA lentivirus. Left panel: western analysis of Sox2, Nf2 and YAP. Upper right panel—Nf2 mRNA expression normalized to actin. Lower right panel- sphere assay. *P<0.05 by t-test. Error bars represent mean ± s.d. (c) Western analysis of Sox2, GFAP and Nf2 in primary GBM cells grown in sphere cultures (sphere) or differentiated for 3, 6 or 12 days (D3, D6 and D12, respectively). (d) Schematic of Sox2 regulation of Hippo pathway components. Sox2 represses Nf2 and WWC1 that restrain YAP transcriptional function.
