Figure 4: S1P3 and Sphk1 mediate in part the effect of histamine on P-selectin mobilization and leukocyte rolling.
From: Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin
(a) HUVECs were stimulated with two histamine concentrations (0.25 and 25 μM) in the presence or absence of SKi (25 μM) or SKi+S1P (1 μM) for 5 min as indicated, and cell surface P-selectin was measured by flow cytometry. Data shown are from three independent experiments. (b) HUVECs were stimulated with histamine (0.25 μM) in the presence or absence of the S1P3 inhibitor TY-52156 (10 μM) and P-selectin was analysed by flow cytometry. Data are from five independent experiments. (c) Leukocyte rolling was quantified in postcapillary venules of the cremaster muscle of Sphk1−/− mice and controls (n=4 per group). P-selectin-blocking antibody was injected to confirm rolling dependence on P-selectin. Quantitative data are presented as mean±s.e.m. Significance was established using a paired two-tailed Student’s t-test or a Wilcoxon rank sum test.*P<0.05; *P<0.05; ***P<0.001.
