Figure 6: Supernatants of activated mast cells stimulate P-selectin mobilization in HUVEC in a S1P3-dependent manner.
From: Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin
(Top) FACS analysis of mouse peritoneal mast cells (CD45+ Lin− CD117+ Sca-1+ CD16/32+ CD34+) used for stimulation. (Bottom) HUVECs were stimulated with supernatants of heat-activated peritoneal mast cells for 10 min in the presence or absence or the S1P3 inhibitor TY-52156 (10 μM). Diphenhydramine and cimetidine (10 μM each) were present in all experiments. P-selectin was measured using flow cytometry. Quantitative data are presented as mean±s.e.m. for three independent mast cell preparations and three independent HUVEC preparations. Significance was established using a paired two-tailed Student’s t-test. P<0.05.
