Figure 6: WEHI-345 desynchronizes NF-κB and AP-1 transcription factors.
From: A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production
(a) THP-1 cells were stimulated with 200 ng ml−1 L18-MDP for the indicated time points, cytoplasmic and nuclear fractions were prepared and probed by western blot with the indicated antibodies. (b) THP-1 NF-κB GFP reporter cells were either left untreated or treated with WEHI-345 (500 nM) 30 min before the addition of L18-MDP (200 ng ml−1). MFI (GFP) was measured at the indicated time points. n=3. Error bars are s.e.m.
