Figure 3: Functional enhancer assays in zebrafish.
From: A PAX1 enhancer locus is associated with susceptibility to idiopathic scoliosis in females
(a) Representative zebrafish (of at least 100 tested for each construct) injected with Xe1-E1b-GFP-Tol2 enhancer construct showing expression predominantly in the somitic muscle. (b) Representative zebrafish injected with PE7-E1b-GFP-Tol2 enhancer construct showing expression predominantly in the somitic muscle. (c) Representative zebrafish injected with PEC7risk-E1b-GFP-Tol2 enhancer construct leads to loss of expression throughout. Corresponding pictures at higher magnification are shown below.
