Figure 5: Control of cardiac-specific profile by Bmi1.
From: Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence
(a) Representative heat maps show the expression of genes with fetal cardiac programme-related genes, senescence-associated and cell-cycle functional annotations that are significantly down- and upregulated in Bmi1f/f;αMHCTM-Cretg/+ (mDCM) and control heart cells (mControl), and hDCM and hControl. The rows correspond to genes and the columns to samples. Gene expression values (relative to the mean expression in control cells) are indicated on a log2 scale according to a colour scheme shown. (b) ChIP analyses of the promoter regions in the indicated genes from total heart cells from Bmi1f/f;αMHCTM-Cretg/+ mice and littermate controls. Chromatin-bound DNA was probed with antibodies to Bmi1, H3K27me3, H2AK119 and H3K4me3. Percentages of input DNA are shown as the means±s.d. of triplicate independent experiments (*P<0.05; Student’s t-test; Student’s t-test).
