Figure 1: Ascl1 directly regulates Cenpjexpression in the embryonic telencephalon. | Nature Communications

Figure 1: Ascl1 directly regulates Cenpjexpression in the embryonic telencephalon.

From: Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1

Figure 1

(a) Illustration of the Ascl1-binding signal in E14.5 telencephalic cells obtained by chromatin immunoprecipitation with promoter microarrays (chromatin immunoprecipitation (ChIP)-chip). The plot displays the ChIP enrichment ratio for Ascl1 (red) and control (black) samples in promoter regions. Black arrow indicates the transcription start site and direction of transcription. (b,c) Western blot analysis of Cenpj protein levels extracted from E14.5 Ascl1 mutants compared with wild-type (WT) telencephalon. Data presented as mean±s.e.m., n=3, Student’s t-test, *P<0.05. (d,e) Immunohistochemistry for Cenpj on coronal sections of WT and Ascl1 mutant E14.5 embryos. The Cenpj protein (d) is localized with the centrosome marker centrin in apical cortical progenitors and the signal is reduced in Ascl1 mutant progenitors (e). Scale bars, 1 μm. (f) Quantitative PCR analysis of Cenpj transcripts extracted from E14.5 Ascl1 mutants compared with WT telencephalon. Data presented as mean±s.e.m., n=3, Student’s t-test, *P<0.05. (g–i) In situ hybridization for Cenpj on coronal sections of the developing telencephalon at E14.5 from WT (g,i) and Ascl1 knockout mouse (h) using an antisense Cenpj probe (g,h) and a control Cenpj sense probe (i). Expression of Cenpj was reduced in the ventricular zone and cortical plate of the Ascl1 mutant cortex. Scale bar, 100 μm. DAPI, 4',6-diamidino-2-phenylindole.

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