Figure 1: Vδ gene segment usage among Vγ1⁺ and Vγ4⁺ cells from peripheral lymphoid organs.

(a) Schematic presentation of a rearranged Trd locus and primers for RACE PCR (O: SMARTer anchor oligonucleotide; V: variable, D: diversity, J: joining and C: constant gene segments). (b,c) Results of RACE PCR product sequencing. Bars indicate proportion of in-frame rearrangements of Vδ gene segments within the purified Vγ1⁺ or Vγ4⁺ cells pooled from the pLN and spleen of three TcrdH2BeGFP mice. (*: Vδ3 is a pseudogene). For each Vγ pool, at least 22,000 in-frame sequences were analysed. Data are representative of two independent experiments that gave similar results.