Figure 2: One Vδ5Dδ2Jδ1 sequence is dominant in CCR6⁺CD27⁻ Vγ4⁺ T cells.

(a) Schematic presentation of a rearranged Trd locus showing the position of representative primers used for amplification of variable Trd regions between Vδ5 and Jδ1, F: forward primer, R: reverse primer. (b) CDR3 amino-acid sequence length distribution and composition for in-frame rearrangements of Vδ5–Jδ1 chain in Vγ1⁺ T cells (left panel), and Vγ4⁺ T cells (middle panel) isolated from a pool of pLN and spleen, or Vγ7⁺ intestinal IELs (right panel), of three TcrdH2BeGFP mice in each subset. (c) In-frame rearrangements of Vδ5–Jδ1 chains in Vγ4⁺ T cells pooled from the pLN and spleen of four TcrdH2BeGFP mice (wt; left panel) or four tamoxifen-induced Indu-Rag1 x TcrdH2BeGFP mice (right panel). Genomic DNA from 10,000 Vγ4⁺ T cells was used as template. For each subset, 5,870 sequences were analysed. (d) In-frame rearrangements of Vδ5–Jδ1 chains in CCR6⁻CD27⁺ (left panel) versus CCR6⁺CD27⁻ (right panel) Vγ4⁺ T cells pooled from the pLN and spleen of three TcrdH2BeGFP mice. cDNA from 4,800 CCR6⁻CD27⁺ or CCR6⁺CD27⁻ Vγ4⁺ cells was used as template. For each subset, 3,552 sequences were analysed. Individual in-frame-rearranged CDR3 amino-acid sequences are separated by colours. Data are representative of at least two independent experiments that gave similar results.