Figure 5: TCR diversity of CCR6⁻CD27⁺ versus CCR6⁺CD27⁻ Vγ4⁺ cells.

(a) Proportion of 309 individual sequences of CCR6⁻CD27⁺ Vγ4⁺ cells, or 276 individual sequences of CCR6⁺CD27⁻ Vγ4⁺ cells, among 3,552 Vδ5 sequences of each subset. Each individual sequence is counted only once regardless of abundance. (b) Proportion of total sequences from the same pool as a, considering the abundance. For a,b, data shown are from one representative of two independent experiments. (c) Diversity measured as ‘effective number of sequences’ (e to the power of the Shannon index) for the same data set of 3,552 CDR3 amino-acid sequences in each subset, including the canonical sequence (light blue), or after eliminating the canonical sequence from the repertoires (dark blue). (d) Venn diagrams display overlap between the 100 most frequent CDR3 amino-acid sequences in similar populations from two independent experiments. Numbers indicate overlapping individual sequences. Similarity between the repertoires from two independent experiments was calculated using the Morisita–Horn index (MH). The MH index reveals the degree of similarity by analysing individual sequences considering their frequency among the total repertoire, scoring from 0 for completely dissimilar subsets to 1 for identical subsets.