Figure 2: Involvement of Tpr in HIV-1 replication.
From: Chromatin organization at the nuclear pore favours HIV replication
(a) HeLa P4CCR5 cells were depleted for Tpr using different doses of LVshRNA against Tpr and (b) infectivity was measured 48 h p.i. by flow cytometry (c) Jurkat cells were depleted for Tpr using different doses of LVshRNA against Tpr, as shown in the western blot and (d) infectivity was measured 48 h p.i. by luciferase activity normalized to the amount of protein. (e,f,g) Viral late reverse transcription (LRT) levels were analysed at 7 h p.i., nuclear import and integration were analysed at 24h p.i. by 2LTRs and Alu-PCR, respectively. Infections carried out in the presence of Nevirapine at 5 μM led to undetectable levels of LRT, 2LTR circles and Alu-PCR. Similar results were obtained in three independent experiments and s.d. are shown.
