Figure 3: Tpr is specifically involved in HIV-1 infection.
From: Chromatin organization at the nuclear pore favours HIV replication
(a) Three out of ~10 final clones obtained by limiting dilution were selected for the expression of Tpr by western blotting and (b) MLV-Luc and HIV-1-Luc were used in parallel to infect the control clone (obtained with an empty LV) and clones 2, 5 and 6. (c) Tat was transfected in all clones to evaluate LacZ expression by β-galactosidase assay normalized to the amount of protein. Clones were thawed and frozen multiple times and yielded similar results (s.d. are shown). (d–f) Transcriptome profiles comparison: scatter plots show RPKM values observed in ~36,000 annotated in control sample (x axis) and Tpr KD (y axis). Scatter plots of Pearson correlation calculated using the RNA-seq log2 RPKM values of Tpr depleted versus control for (d) HeLa cells, (e) Jurkat cells and (f) clones.
