Figure 1: Notch activation links major neural lineage transitions in hESC derived neural progenitor cells. | Nature Communications

Figure 1: Notch activation links major neural lineage transitions in hESC derived neural progenitor cells.

From: Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors

Figure 1

(a) Neural differentiation scheme. Neural induction was performed by a dual SMAD inhibition protocol followed by long-term propagation with the factors indicated for 220 days. Naming conventions representing neuroepithelial (NE), early radial glial (E-RG), midradial glial (M-RG), late radial glial (L-RG) and long-term cultured progenitors (LNP) are indicated. Number of passages are indicated as P(n). (b) Bright field microscopy of progenitor cells during long-term differentiation shows dynamic morphological features. Scale bar: 50 μm (valid for all images in b). (GFP matched images can be seen in Supplementary Fig. 1a). (c) Combined HES5::eGFP reporter expression and Immunostainings of stem/progenitor cell as well as differentiation markers throughout the progression period. Top: PAX6, SOX1 and HES5 induction during early stages (See also Supplementary Fig. 1a,b for HES5::eGFP percentages). Middle and Bottom: SOX2, NESTIN and DCX expression. Scale bar: 50 μm (valid for all images in c). Individual qPCR analyses for all genes tested at all stages are shown in Supplementary Fig. 1d.

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