Figure 5: Glial transformation with respect to Notch activation. | Nature Communications

Figure 5: Glial transformation with respect to Notch activation.

From: Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors

Figure 5

(a) Combined HES5::eGFP reporter expression and immunostainings of the RG markers GLAST (top) and FABP7 (bottom). Scale bar: 50 μm (valid for all images in a). (b) High-power magnification of E-RG and M-RG images for selected genes shown in a. Scale bar: 25 μm (valid for all images in b). (c) EGFR expression percentages by FACS analysis for L-RG (purple) and LNP (turquoise) stages is shown. Average of 2 independent experiments is shown. Statistical analysis: mean±s.e.m. (d) Relative GFAP expression levels based on qPCR data for the entire progression period. Relative expression levels are shown for HES5+ and HES5− samples during progenitor proliferation. Values were obtained from three technical replicates. Statistical analysis: mean±s.e.m. Compare the very low relative levels of GFAP during proliferation (day 80 HES5+ cells) to GFAP levels at the same progenitor type following astrocytic differentiation in Fig. 3e.

Back to article page