Figure 6: Rab5 and dynamin are required for Ca2+ transients initiation.
From: Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons
(a) Long-term time traces of GCaMP6s fluorescence in control (top), Rab5-DN expressing (middle) and Shi-DN expressing neurons (bottom). Fluorescence changes in two rectangle regions are shown. (b) Quantification of the percentage of C4da neurons with dendrites producing Ca2+ transients at 7 h a.p.f.. **P<0.001, χ2-test followed by Ryan’s test. (c) Scheme of the temperature shift experiment. Animals expressing or not expressing Shi-ts in C4da neurons are raised at 22 °C during the entire larval stage, and at the permissive (25 °C) or non-permissive (33 °C) temperature from the onset of metamorphosis. Ca2+ imaging is performed at 7 h a.p.f.. (d) Quantification of the percentage of C4da neurons with dendrites producing Ca2+ transients at 7 h a.p.f.. **P<0.001 comparing with all other groups, Fisher’s exact test.
