Figure 1: GPC3 expression and internalization in HCC cells. | Nature Communications

Figure 1: GPC3 expression and internalization in HCC cells.

From: Immunotoxin targeting glypican-3 regresses liver cancer via dual inhibition of Wnt signalling and protein synthesis

Figure 1

(a) Flow cytometric results, detecting cell surface GPC3 expression in the GPC3-positive cell lines HepG2, Hep3B, Huh-1 and Huh-7. Cell line A431 does not express GPC3, and is shown as a negative control. G1 cells that overexpress GPC3 in the A431 cell line is shown as a positive control. Shaded grey peaks represent the cell surface staining with isotype control; white peaks represent the cell surface staining of GPC3. (b) Western blot analysis of endogenous GPC3 expression in HCC and other cell lines. For each sample, 20 μg total protein was loaded for detection. (c) Quantification of GPC3 sites per HCC cell using QuantiBrite PE beads. (d) Flow cytometric detection of internalization rates of GPC3. Each western blot and flow cytometric results representative of at least three independent experiments. MW, molecular weight.

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