Figure 2: Affinity and cytotoxicity of HN3-PE38 and YP7-PE38. | Nature Communications

Figure 2: Affinity and cytotoxicity of HN3-PE38 and YP7-PE38.

From: Immunotoxin targeting glypican-3 regresses liver cancer via dual inhibition of Wnt signalling and protein synthesis

Figure 2

(a) ELISA and flow cytometric analysis of HN3-PE38- and YP7–PE38-binding-affinities for GPC3 protein and G1 cells. The results are representative of at least three independent experiments. (b) Inhibition of cell proliferation on different cell lines by HN3-PE38 and YP7-PE38 immunotoxins, determined by a WST-8 assay. SK-Hep-1 was showed as an antigen-negative cell line. Dashed line indicated the value of IC50. IT, immunotoxin. Values represent mean±s.d. (c) Cytotoxicity of HN3-PE38 on wild type or GPC3 knocked down Hep3B and HepG2 cells. Western blot showed knockdown efficiency. wt, wide type; kd, knockdown. Dashed line indicated the value of IC50. Values represent mean±s.d. MW, molecular weight.

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