Figure 3: Construction and analysis of inactive anti-GPC3 immunotoxins. | Nature Communications

Figure 3: Construction and analysis of inactive anti-GPC3 immunotoxins.

From: Immunotoxin targeting glypican-3 regresses liver cancer via dual inhibition of Wnt signalling and protein synthesis

Figure 3

(a) Flow cytometric analysis of active and inactive HN3-PE38- and YP7-PE38-binding affinities for G1 cells. The KD values for G1 cells were based on mean fluorescence intensity (MFI). (b) [3H] Leucine incorporation assay to detect protein synthesis on Hep3B cells treated with active or inactive anti-GPC3 immunotoxins. Dashed line indicated the value of IC50. Values represent mean±s.d. (c) Cytotoxicity of active and inactive HN3-PE38 and YP7-PE38 on Hep3B cells, determined by a WST-8 assay. Dashed line indicated the value of IC50. Values represent mean±s.d.

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