Figure 4: Inhibition of Wnt3a-induced β-catenin and Yap signalling by inactive HN3-PE38. | Nature Communications

Figure 4: Inhibition of Wnt3a-induced β-catenin and Yap signalling by inactive HN3-PE38.

From: Immunotoxin targeting glypican-3 regresses liver cancer via dual inhibition of Wnt signalling and protein synthesis

Figure 4

(a) Topflash activity of HEK293 Topflash cells treated with 0.5 μg ml−1 inactive HN3-PE38 and YP7-PE38 in the presence of Wnt3a. Inactive HS20-PE38 and active irrelevant IT were set up as a positive and negative control, respectively. IT, immunotoxin. Values represent mean±s.d. **P<0.01 compared with Wnt3a-treated group, Student’s t-test. (b) Active β-catenin expression on HEK293 Topflash cells treated as described in a for 12 h. (c) Yap/TEAD activity of Hep3B cells treated with 10 μg ml−1 inactive HN3-PE38 and YP7-PE38 in the presence of Wnt3a for 16 h. Values represent mean±s.d. **P<0.01 compared with Wnt3a-treated group, Student’s t-test. (d) Cytotoxicity of HN3-PE38 and YP7-PE38 on wild type and Yap knockdown Hep3B cells. Data were presented as fold change of IC50 (wt versus kd). Western blot showed knockdown efficiency. Values represent mean±s.d., *P<0.05 and **P<0.01 compared with HN3-PE38-treated group, Student’s t-test. wt, wide type; kd, knockdown; MW, molecular weight.

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