Figure 2: Impact of Id4 deficiency on ductal morphogenesis and proliferation.
From: ID4 controls mammary stem cells and marks breast cancers with a stem cell-like phenotype
(a) Whole-mount staining of Id4+/− and Id4+/+ mammary glands at 8 weeks of age. Graphs indicate the mean±s.e.m. Id4+/− n=8, Id4−/− n=7. ***P<0.0005, unpaired t-test. Scale bars, 1 mm. (b) Analysis of the ability of epithelium from Id4−/− and Id4+/− mice to fill a mammary fat pad. Graphs indicate the mean±s.e.m. Id4+/− n=6, Id4−/− n=5. *P<0.05, unpaired t-test. Scale bars, 1 mm. (c) Western blot following Id4 knockdown by two independent shRNA (shId4 #1 and shId4 #2) compared with a scrambled control (shCont) and GFP-transduced Comma-D cells (for full western blot see Supplementary Fig. 9). (d) Proliferation assays by cell counting following Id4 knockdown using two independent shRNA constructs and two independent controls (shControl and shGFP). (n=3 independent experiments) **P<0.0002, two-way ANOVA.
