Figure 4: Effect of identified amino-acid substitutions in the NA, PB1 and NP on protein function and replication kinetics of recombinant A/Anhui/1/2013 (H7N9) influenza viruses in vitro.
From: Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck
(a) Effect of temperature on polymerase complex activity with the indicated amino-acid substitutions. (b) Effect of the indicated amino-acid substitutions on NA activity. (c) Cell surface expression of the NAs shown in panel b. (d–f) Replication kinetics of rg-A/Anhui/1/2013 (H7N9) influenza viruses with the indicated amino-acid substitutions in the NA, PB1 and NP genes in MDCK cells. Values are mean and s.d. from two independent experiments (n=4). *P<0.05 compared with WT virus by two-way analysis of variance.
