Table 2 The effect of the identified substitutions in the NA and polymerase genes on the total amount of influenza virus produced over the course of the experiment using replication kinetic assays in MDCK cells.

From: Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck

rg-A/Anhui/1/2013 (H7N9) influenza virus

AUC0–24 (mean log10TCID50 per ml−1±s.d.)

P value*

WT

80.38±3.11

NA

NAE73K

63.88±2.29

<0.05

NAI300V

76.20±2.26

0.07

PB1D76N

81.10±3.79

0.78

NPI365V

77.19±2.20

0.15

NAE73K+ NAI300V

71.13±2.07

<0.05

PB1D76N+ NPI365V

72.25±1.67

<0.05

NAE73K+ NAI300V+PB1D76N+ NPI365V

69.19±3.74

<0.05

  1. NA, not applicable.
  2. AUC0–24,the area under virus titre hour curve from 0 to 24 h post inoculation. Values are mean and s.d. from two independent experiments (n=4).
  3. *P values were compared with WT virus by two-way analysis of variance.
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