Figure 1: Characteristics of leader cells in collective cell migration. | Nature Communications

Figure 1: Characteristics of leader cells in collective cell migration.

From: Notch1–Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration

Figure 1

(a) Schematic representation of a migration tip with leader–follower organization during collective cell migration. Leader cells (green) at the front of the leading edge typically display enlarged cell size, ruffling lamellipodium, large focal adhesions and aligned cytoskeletal architecture. (b) Representative immunofluorescence image of F-actin (red) and vinculin (green) in leader cells formed at the leading edge. (c–e) Actin stress fibres in cells transfected with dsLNA probes targeting (c) β-actin mRNA, (d) Dll4 mRNA and (e) a random sequence. Cells were first transfected with the dsLNA probes (green) and fixed for immunostaining (red). (f–h) Focal adhesion in cells transfected with dsLNA probes targeting β-actin mRNA (f), Dll4 mRNA (g) and a random sequence (h). Samples were counterstained with DAPI (blue). Images are representative of three independent experiments. Scale bars, 50 μm. (i–j) HE staining of epithelial cells in skin punch wounds. (k–m) IHC staining of IgG control (k), Dll4 (l) and Notch1 (m). Yellow dotted lines indicate the epithelium and black arrows indicate the wound boundaries. Scale bars, 200 μm.

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