Figure 2: Dynamics of Dll4 expression and leader cell formation during collective migration. | Nature Communications

Figure 2: Dynamics of Dll4 expression and leader cell formation during collective migration.

From: Notch1–Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration

Figure 2

(a) Time-lapse fluorescence images characterizing Dll4 mRNA expression. Cells were transfected with dsLNA probes targeting Dll4 mRNA. The model wound was created by scratching the monolayer with a sterilized 1,000 μl pipette tip. Scale bars, 50 μm. Representative cells were labelled with numbers (1–7). (b,c) Tracing of Dll4 mRNA expression in representative cells in a illustrating the dynamic behaviours and competitive characteristics of leader cells in collective migration (n=3). Pixel intensity data are expressed as the mean±s.e.m.

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