Figure 3: Notch1–Dll4 signalling regulates leader cell formation in collective cell migration. | Nature Communications

Figure 3: Notch1–Dll4 signalling regulates leader cell formation in collective cell migration.

From: Notch1–Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration

Figure 3

(a–c) Double immunofluorescence staining of Notch1 and Dll4 in model wounds (top). For Dll4 mRNA detection, cells were first transfected with the dsLNA probes before fixation for Notch1 and Dll4 immunostaining (middle and bottom). (a) Notch1 nuclear translocation and Dll4 expression in model wounds. (b,c) Effects of DAPT (b) and Jagged-1 (c) on Notch1 and Dll4 proteins and Dll4 mRNA. (d–f) Effects siRNA knockdown on Notch1 and Dll4 and Dll4 mRNA. Control siRNA (d), Notch1 siRNA (e) and Dll4 siRNA (f) were transfected to the cells 48 h before wounding. Samples were counterstained with DAPI (blue). Images are representative of three independent experiments. Scale bars, 100 μm.

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