Figure 6: SUV39H1 inhibits pol III loading and expression of some SINEs.
(a) Mean±s.e.m. of the percentage input bound in two independent ChIP–quantitative PCR (qPCR) assays with HeLa cells, showing occupancy of H3, TAFI48, H3K9me3, SUV39H1 and HP1 at Alu loci, 7SL and p21 (positive control) genes. (b) Mean±s.e.m. of the percentage input bound in two independent ChIP–qPCR assays with HeLa cells treated for 24 h with vehicle (−) or 100 nM chaetocin (+), showing occupancy of H3, TAFI48, H3K9me3, pol III and TFIIIC at Alu loci, 7SL and Apo-E genes. (c) Semiquantitative RT–PCR analysis of expression levels of indicated transcripts in HeLa cells treated for 24 h with indicated concentrations of chaetocin. (d) Analysis by primer extension of expression levels of Alu transcripts initiated from pol III start site in HeLa cells treated for 24 h with indicated concentrations of chaetocin. Reverse transcriptase was omitted from reactions in lanes 5–8. Right panel shows mean±s.d. of fold change in Alu expression in two independent experiments quantified using ImageJ. All P values are calculated by t-test.
