Figure 2: ClustalW alignment of representative bacterial MddA polypeptides and the Saccharomyces cerevisiae Ste14p polypeptide.
From: A novel pathway producing dimethylsulphide in bacteria is widespread in soil environments
Residues that are identical or have similar properties are highlighted in red, orange or yellow if they are conserved in all nine, at least seven or at least five polypeptides, respectively. Residues with similar properties that are conserved in all eight bacterial MddA sequences are marked with asterisks below. The position of the Tn5lacZ insertion in the Pseudomonas deceptionensis M1T mddA mutant J566 is marked with an ‘X’ below. Species names with the MddA polypeptide accession codes shown in brackets are: Cyanothece sp. ATCC 51142 (YP_001803274), Mycobacterium tuberculosis H37Rv (NP_217755), Bradyrhizobium diazoefficiens USDA 110 (Blr1218; NP_767858.1), Bradyrhizobium diazoefficiens USDA 110 (Blr5741, NP_772381.1), Pseudomonas sp. GM41 (WP_008148420), Pseudomonas deceptionensis M1T (AJE75769), Maricaulis maris MCS10 (YP_757811.1), Sulfurovum sp. NBC 37-1 (YP_001358232.1). Species names marked with a hash indicate those MddA sequences whose activity has been ratified in Escherichia coli. The Saccharomyces cerevisiae Ste14p accession is AAA16840.1.
