Figure 1: Rgs1 is upregulated by inflammatory stimuli in activated monocytes.

(a) Confirmation of Rgs1 mRNA changes in thoracic aortas of ApoE^−/− mice and wild-type controls on a high-fat diet (n=5 per group) by qRT–PCR. (b) Rgs1 expression is correlated with the expression of the macrophage marker Cd68 in thoracic aortas of ApoE^−/− mice and wild-type controls on a high-fat diet. Each symbol represents an individual mouse (n=5 per group) by qRT–PCR. (c) qRT–PCR analysis of Rgs1 mRNA in primary cells isolated from ApoE^−/− mice (n=5–6; BC, B cells; BM, bone marrow cells; EC, endothelial cells; MΦ, macrophages; S, splenocytes). (d) qRT-PCR analysis of Rgs1 expression in human tissue and cells. (Mo; Blood monocytes, plaque macrophages from carotid endarterectomies (n=3), SV; Saphenous vein and IMA; internal mammary artery from CABGs (n=8), OA; omental artery and AAA; abdominal aortic aneurysm from AAA repair patients (n=8–11)). (e) SV and IMA are from CABGs. qRT–PCR analysis of Rgs1 mRNA in Ly6G-7/4^hi BM monocytes and peritoneal monocytes isolated from zymosan-induced peritonitis (ZIP) in ApoE^−/− mice (n=6–7 per group). (f) qRT–PCR analysis of Rgs1 mRNA in bone marrow-derived macrophages from ApoE^−/− mice stimulated with IFN-γ and lipopolysaccharide (M1) and unstimulated (M0) over 24 h presented relative to mRNA in unstimulated cells, set as 1. *P<0.05, **P<0.01 calculated using the Student’s t-test (Data in a are expressed as mean±s.d. and data in c–f as mean±s.e.m.).