Figure 2: The validity of key steps in the workflow was evaluated by model study of Raji B cells. | Nature Communications

Figure 2: The validity of key steps in the workflow was evaluated by model study of Raji B cells.

From: Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics

Figure 2

(a) The efficiency of dephosphorylation step was evaluated by quantitative comparison of sum of the extracted ion chromatogram (XIC) from phosphopeptides before and after phosphatase treatment. (b) The motif of motif-targeting phosphopeptides after kinase reaction by MAPK and CK2. (c) As shown in the scatter plot of the measured stoichiometry between biological duplicate, correlation coefficient between replicate experiments was R2=0.968 (s.d.=6.1%) for CK2 and R2=0.960 (s.d.=6.4%) for MAPK, respectively.

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