Figure 4: IgG ICs cause enhanced osteoclastogenesis in Fcgr2b−/− mice.
From: Immune complexes regulate bone metabolism through FcRγ signalling
(a) Fractionation of mouse serum isolated from Fcgr2b+/+ and Fcgr2b−/− mice using size-exclusion chromatography. Purified mouse IgG was used as a reference. Fractions with a molecular weight corresponding to IgG and higher (in the dotted rectangle) were used for the analysis in Fig. 2b. Representative data of three independent experiments are shown. (b) ELISA analysis of IgG in the peak fractions (F4, 6, 14). (c) Effect of IgG-containing fractions on osteoclast formation in Fcgr2b−/− cells. (d) Effect of IgG IC generated by mixing the WT mouse serum and a F(ab’)2 segment on osteoclast differentiation of Fcgr2b−/− cells or WT cells. (e) Effect of soluble IC composed of TNP-BSA together with α-TNP IgG1 (IgG1 IC) on osteoclast differentiation in WT, Fcgr2b−/− and Fcgr2b−/−Fcer1g−/− cells. All quantification experiments were performed using triplicate culture wells. All data are representative of more than three independent experiments and are shown as the mean±s.e.m. Statistical analyses were performed using unpaired two-tailed Student’s t-test (*P<0.05; **P<0.01; ***P<0.001; n.s., not significant).
