Figure 3: In vivo protein import defects. | Nature Communications

Figure 3: In vivo protein import defects.

From: Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances

Figure 3

(a) Immunoblots showing the steady-state levels of ATOM40, VDAC, CoxIV, cytochrome c1 (Cyt c1), MCP5, mtHSP70 and REAP1 in whole-cell extracts of the indicated knockdown cell lines. Cytosolic EF1a serves as a control. Time of induction in days (d) is indicated at the top. Black triangles indicate the onset of the growth phenotype. The position of precursor (p) and mature forms (m) are indicated. (b) Immunoblot analysis of whole-cell (T), digitonin-extracted, mitochondria-enriched pellet (P) and soluble (S) fractions of the indicated knockdown cells. VDAC and EF1a serve as markers for mitochondria and cytosol, respectively. (c) As in a but results are for ATOM69.

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