Figure 4: In vitro protein import defects.

35S-Met-labelled LDH–DHFR was imported into mitochondria isolated from the indicated uninduced and induced knockdown cell lines. All import reactions were treated with proteinase K and analysed by SDS–PAGE followed by autoradiography. Ψ, membrane potential. Input, 10% of the added substrate, Coomassie-stained gels are shown as loading controls. The position of precursor (p) and mature forms (m) are indicated.