Figure 5: ATOM complex architecture and functional interactions between its subunits. | Nature Communications

Figure 5: ATOM complex architecture and functional interactions between its subunits.

From: Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances

Figure 5

(a) BN–PAGE immunoblots of mitochondrial membrane extracts from wild-type cells were probed with antisera against the indicated subunits. For ATOM12, a c-Myc tag version of the protein was analysed. Quantifications of lane profiles revealed the presence of four high molecular weight complexes termed: core, A, B and C. Molecular weight markers (kDa) are indicated. (b) Model of the composition of the complexes. Arrows indicate the suggested assembly pathway. (c) Top panels, SDS–PAGE immunoblot analysis of steady-state levels of individual ATOM complex subunits in all knockdown cell lines. Left and right lanes represent uninduced and induced cell lines, respectively. Lower panel, summary of the immunoblotting data (see also Supplementary Fig. 4).

Back to article page