Figure 1: Identification of Foxc1 as a candidate transcription factor selectively expressed in chondrocytes.
From: The transcription factor Foxc1 is necessary for Ihh–Gli2-regulated endochondral ossification
(a,b) Total RNA isolated from Venus-negative and -positive cells was analysed by RT–qPCR for chondrocyte marker genes (a) and Foxc1 (b) expression. Data are shown as the mean±s.d. (n=3). **P<0.01 (versus Venus (−)); Student’s t-test. (c) Tissue distribution of Foxc1 (upper panel) and Col2a1 (lower panel) mRNA in newborn mouse tissues. Total RNA isolated from indicated tissues was analysed by RT–qPCR. Data are shown as the mean±s.d. (n=3). (d) The expression of Foxc1 and Col2a1 in developing limbs of E12.5 mice was determined by whole-mount in situ hybridization. Scale bar, 2 mm (left panel), 1 mm (right panel). (e) Haematoxylin and eosin (H&E) staining and in situ hybridization analysis of Col2a1 (middle panel) and Foxc1 (right panel) in growth plate chondrocytes of E15.5 mice tibia. The boxed area shows higher magnification of distal chondrocytes. Scale bar, 200 μm (lower magnification, × 20) and 100 μm (higher magnification, × 40).
