Figure 1: Overall shape of Sti1 and Sti1 fragments.
From: Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules
(a) Scheme of Sti1 and Sti1 deletion variants investigated in this study. (b) Molecular mass of Sti1 variants as calculated assuming globular proteins (grey) and experimentally derived from analytical gel filtration experiments (black). The differences between these indicate that Sti1 fragments containing the linker exhibit an elevated hydrodynamic radius and possess an elongated shape in solution. (c,d) Dmax (left) and Rg (right) distributions of the ensemble optimization method models of wild-type Sti1 and mutants in the initial pool of structures with randomized interdomain linkers (dashed lines) and in the selected ensembles (solid lines).
