Table 1 SAXS data and analysis.
From: Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules
Sample | Stoichiometry | R g (Å) | D max (Å) | Molecular mass (kDa)* |
|---|---|---|---|---|
Sti1 | — | 58.4±0.6 | 264 | 70 |
Sti1 Δlinker | — | 50.1±0.5 | 207 | 60 |
Sti1–ΔTPR1 | — | 54.0±0.1 | 200 | 48 |
Sti1–TPR2A–TPR2B–DP2 | — | 36.0±0.1 | 130 | 35 |
Sti1–ΔDP2 | — | 60.3±0.1 | 220 | 58 |
Sti1–TPR1–DP1 | — | 30.4±0.1 | 100 | 29 |
Sti1–TPR2B–DP2 | — | 23.2±0.1 | 80 | 17 |
Sti1–TPR2A–TPR2B | — | 33.9±0.1 | 130 | 24 |
Hsp70 | — | 40.0±0.1 | 140 | 70 |
Sti1+Hsp70 | 1:1 | 71.2±0.1 | 240 | 135 |
1:2 | 76.2±0.1 | 280 | 123 | |
Sti1 Δlinker+Hsp70 | 1:1 | 60.1±0.1 | 200 | 138 |
1:2 | 59.7±0.1 | 200 | 100 | |
Sti1–TPR1+Hsp70 | 1:1 | 45.0±0.2 | 160 | 86 |
Sti1–TPR2B+Hsp70 | 1:1 | 45.7±0.1 | 170 | 80 |
