Table 3 FRET efficiency values extracted from the HMM analysis of the TIRF measurements.

From: Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules

 

Low

Intermediate

High

G193C—G309C

 + Hsp70

0.16

0.52

0.84

 + Hsp90

0.24

0.66

0.92

 + Hsp70+Hsp90

0.23

0.57

0.86

G193C—G309C N39A

 + Hsp70

0.18

0.62

0.92

 + Hsp90

0.10

0.58

0.91

 + Hsp70+Hsp90

0.14

0.58

0.91

G193C—G309C N435A

 + Hsp70

0.18

0.65

0.93

 + Hsp90

0.20

0.57

0.92

 + Hsp70+Hsp90

0.17

0.57

0.92

  1. FRET=Förster resonance energy transfer; HMM=Hidden Markov model; TIRF=total internal reflection fluorescence microscopy.
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