Figure 3: Il17rd ^−/− mice are more susceptible to TLR-induced lethality.

(a) Survival of age- and sex-matched WT and Il17rd^−/− mice (n=22) injected i.p. with LPS (20 mg per kg body weight) over 96 h. (b) Sandwich ELISA of IL-6, KC and the IL-12/IL-23 p40 subunit in serum of age- and sex-matched WT and Il17rd^−/− mice (n=10–12) measured 16 h after i.p. injection with LPS (20 mg kg⁻¹). (c) Quantitative PCR (qPCR) analysis of Inos, Tnf and Il6 mRNA levels in spleens from WT and Il17rd^−/− mice (n=10–15) 16 h after i.p. injection with LPS. (d) Survival of age- and sex-matched WT and Il17rd^−/− mice (n=22) injected i.p. with poly(I:C) (0.01 μg per g body weight) and D-galactosamine (0.7 mg per g body weight) over 24 h. (e) Representative images of haematoxylin and eosin staining of liver from surviving WT and Il17rd^−/− mice injected i.p. with poly(I:C) and D-galactosamine. Images were captured at magnifications of × 20 (upper panels: scale bar, 100 μm) and × 40 (lower panels: scale bar, 50 μm). (f) Serum of age- and sex-matched WT and Il17rd^−/− mice (n=10–12) after i.p. injection with poly(I:C) (5 mg per kg body weight) for 16 h was measured for IFN-β, RANTES, IL-6 and the IL-12/IL-23 p40 subunit levels by ELISA. (g) Expression of bioactive type-I interferon was measured by blue sensor cells. (h) qPCR analysis of Cxcl10 and Ccl5 mRNA levels in spleen samples from WT and Il17rd^−/− mice (n=16) 16 h after i.p. injection with poly(I:C) (5 mg kg⁻¹). Data are (e) representative of n=9, or presented (b,c,f–h) as the mean ±s.e.m. of three independent experiments and were subjected to a two-tailed unpaired Student’s t-test, *P<0.05; **P<0.01; ***P<0.001, or (a,d) the log-rank test (PBS/D-GAL n=10) (LPS/poly(I:C) n=22).