Figure 5: Deletion of FoxOs in skeletal muscle partially prevents atrophy during denervation. | Nature Communications

Figure 5: Deletion of FoxOs in skeletal muscle partially prevents atrophy during denervation.

From: Regulation of autophagy and the ubiquitin–proteasome system by the FoxO transcriptional network during muscle atrophy

Figure 5

(a) Frequency histograms of gastrocnemius muscles from FoxO1,3,4−/− and control mice showing the distribution of cross-sectional areas (μm2) of FoxO1,3,4f/f (black bars) and FoxO1,3,4−/− (magenta bars) in control (upper panel) or in denervation (lower panel). n=4 muscles each groups. (b) Force measurements preformed ex vivo on soleus muscles show that FoxO1,3,4−/− muscles are stronger than controls, both in basal condition and after 14 days from denervation. n=6 muscles in each group. (c) Force/frequency curves of denervated soleus highlight the higher strength generated by FoxO1,3,4−/− muscles when compared with controls. n=6 muscles in each group. (d) Immunoblots of gastrocnemius protein extracts reveal a decrease of AKT phosphorylation both in contralateral and in denervated muscles of FoxO1,3,4−/− mice. The increase of 4EBP1 protein is blunted in FoxOs knockout mice. (e) Immunoblots of autophagy-related proteins. FoxOs are required for p62 induction, while LC3 is less lipidated after 3 days of denervation. Data are representative of three independent experiments. Data are shown as mean±s.e.m. Error bars indicate s.e.m. *P<0.05, **P<0.01 (Student’s t-test). C, control; D, denervated.

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