Figure 2: Transcriptome and epigenome profiling in 11 melanoma cell cultures.
From: Decoding the regulatory landscape of melanoma reveals TEADS as regulators of the invasive cell state
RNA-seq, FAIRE-seq and ChIP-seq against H3K27Ac and H3K27me3 across 10 short-passage melanoma cultures and one melanoma cell line SK-MEL-5. The SOX10 gene shows high expression and its upstream regions contain high H3K27ac and FAIRE but low H3K27me3 signal in the nine proliferative (blue) samples. In the two invasive (orange) samples, there is no SOX10 expression, no H3K27Ac and FAIRE peaks but high H3K27me3 peaks. Upper panel shows one invasive sample (MM047) and one proliferative sample (MM011). Lower panels showing zoom in around the promoter region of SOX10 with tracks for all 11 samples for each of the four data types. Vertical axes represent normalized coverage for each data track. Arrows indicate regions of interest that are different between proliferative and invasive states. Other genes are illustrated in Supplementary Figs 4 and 5 and in the UCSC Genome Browser using our Melanoma Track Hub (see Methods).
