Figure 2: Tuning of plasma alanine concentration via GR-mediated skeletal muscle protein catabolism.
From: A muscle-liver-fat signalling axis is essential for central control of adaptive adipose remodelling
(a–c) Blood glucose (a), plasma adrenocorticotropic hormone (ACTH, b) and corticosterone (c) levels of 20-week-old male GRf/f (blue) and GRmKO (red) mice after fasting for the indicated time periods. Error bars represent mean±s.e.m. (n=8). *P<0.05 determined by two-tailed Student’s t-test for unpaired data. (d) Expression levels of the indicated mRNA in Gas (gastrocnemius) from 20-week-old male GRf/f, GRmKO and adrenalectomized C57BL/6J (adrenalectomy (ADX)) mice after fasting for the indicated time periods. Data are normalized to 36B4 mRNA levels and are shown as fold induction to expression levels in fed (0 h) GRf/f mice. Error bars represent mean±s.e.m. (n=8). *P<0.05 determined by two-tailed Student’s t-test for unpaired data. (e) Weight of skeletal muscles from ad libitum-fed and 48 h-fasted 20-week-old male GRf/f and GRmKO mice. Error bars represent mean±s.e.m. (n=12). *P<0.05; **P<0.01 determined by two-tailed Student’s t-test for unpaired data. (f,g) Plasma alanine levels of 20-week-old male GRf/f (blue) and GRmKO (red) mice, which were ad libitum-fed (f) and fasted for the indicated time periods from zeitgeber time (ZT) 12:00 (g). Error bars represent mean±s.e.m. (n=4). *P<0.05 between GRf/f and GRmKO determined by two-tailed Student’s t-test for unpaired data.
