Figure 3: Inefficient energy production in GR-ablated skeletal muscle during fasting.

(a) Intramuscular levels of alanine, pyruvate, glucose, glycogen, triglyceride and branched chain amino acids (BCAA) in Gas (gastrocnemius) from fed or 24 h-fasted 20-week-old male GRf/f (blue) and GRmKO (red) mice. Error bars represent mean±s.e.m. (n=6). *P<0.05 determined by two-tailed Student’s t-test for unpaired data. (b,c) Maximum grip strength of limbs (b) and endurance in wire-hang test (c) of fed or 24 h-fasted 20-week-old male GRf/f (blue) and GRmKO (red) mice are shown as boxplots (n=15). (d) Endurance in forced treadmill exercise of fed or 24 h-fasted 20-week-old male GRf/f (blue) and GRmKO (red) mice are shown as latency to cease running (left panel) and travelled distance (right panel) in boxplots (n=10). Whiskers show the minimum and maximum of all the data, boxes show one standard deviation above and below the mean of the data, and lines inside the boxes show the median of the data (b–d). *P<0.05 determined by two-tailed Student’s t-test for unpaired data (b–d).