Figure 4: Reduced lipid storage in GRmKO mice.

(a) Representative CT transverse section images of ad libitum-fed 30-week-old male GRf/f and GRmKO mice. Retroperitoneal and subcutaneous fat depots, and the 4th lumbar vertebrae are indicated as r, s and L4, respectively. (b,c) Representative photographs (b) and weight (c) of retroperitoneal fat depot (left panels, bars represent 1 cm) and intrascapular fat depot (right panels, bars represent 2 mm) from 30-week-old male GRf/f and GRmKO mice. Data are shown as boxplots of ratio to body weight (n=8). Whiskers show the minimum and maximum of all the data, boxes show one standard deviation above and below the mean of the data, and lines inside the boxes show the median of the data. *P<0.05 determined by two-tailed Student’s t-test for unpaired data. (d) Representative images of haematoxylin and eosin staining of retroperitoneal (left panels) and intrascapular (right panels) fat depots from 30-week-old male GRf/f and GRmKO mice. Scale bars represent 100 μm. (e) Adipocyte cross-sectional area (CSA) distribution of retroperitoneal fat depot from 30-week-old male GRf/f (blue) and GRmKO (red) are shown as frequency histograms (left panel, n=200, from three independent animals of each genotype) and boxplots (right panel). Whiskers show the minimum and maximum of all the data, boxes show one standard deviation above and below the mean of the data, and lines inside the boxes show the median of the data. *P<0.05 determined by two-tailed Student’s t-test for unpaired data.