Figure 6: miR-181c regulates PDPK1 expression in brain endothelial cells.
(a) Microarray analysis showing the expression levels of PDPK1 in brain endothelial cells after transfection of miR-181c. The data are represented as log2 value. (b) Expression level of PDPK1 mRNA in brain endothelial cells after the transfection of miR-181c. Error bars represent s.d., Student’s t-test, n=3. (**P<0.01). (c) Western blot analysis of PDPK1 and GAPDH. Proteins were from brain endothelial cells transfected with miR-181c. The lower panel shows the intensity of PDPK1 obtained from the transfection of N.C. siRNA or miR-181c. This experiment was repeated twice. (d) Microarray analysis showing the expression levels of PDPK1 in brain endothelial cells after EV treatment. (e) Expression level of PDPK1 mRNA in brain endothelial cells after the addition of EVs from D3H2LN, BMD2a or BMD2b cells. Error bars represent s.d., Student’s t-test, n=3 (**P<0.01). (f) Western blot analysis of PDPK1 and GAPDH. Proteins were from endothelial cells treated with EVs from D3H2LN, BMD2a or BMD2b cells. The lower panel shows the intensity of PDPK1 obtained from D3H2LN, BMD2a or BMD2b cells. This experiment was repeated twice. Data are representative of at least three independent experiments each (Fig. b,e).
