Figure 4: In vitro imaging of encapsulated cell lines.
From: Label-free in vivo molecular imaging of underglycosylated mucin-1 expression in tumour cells
(a) Bright-field image (× 10) shows individual microcapsules containing MCF10A cells. Scale bar, 500 μm. (b) Averaged CEST spectra of the five cell lines at a saturation field strength (B1)=2.4 μT. (c) Averaged CEST spectra of the five cell lines at B1=3.6 μT. (d) MT-weighted image showing the phantom layout. (e) CEST contrast map at 1.8 p.p.m. with B1=2.4 μT. (f) CEST contrast map at 1.8 p.p.m. with B1=3.6 μT. (g) Statistical comparison of MTRasym (1.8 p.p.m., B1=3.6 μT) for microcapsules with the five cell lines and without cells. Data represent means±s.e. for three independently performed experiments, analysed using one-way ANOVA (F5,14=28.22, P<0.0001) followed by a Tukey-Kramer test for multiple comparisons. Capital letters on top (A–C) indicate groups that were significantly different from all other groups tested at P<0.05. (h) Validation of MUC1 glycosylation levels using immunostaining with an antibody detecting full-length MUC1 (anti-MUC1 antibody). Red=MUC1, blue=nuclei (DAPI). Scale bar, 200 μm.
